Click image to see more details
Product Info Summary
| SKU: | P01475 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho-S6K1 (T421 + S424) RPS6KB1 Rabbit Monoclonal Antibody
View all p70 S6 Kinase/S6K Antibodies
SKU/Catalog Number
P01475
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-S6K1 (T421 + S424) RPS6KB1 Rabbit Monoclonal Antibody catalog # P01475. Tested in WB, IP applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-S6K1 (T421 + S424) RPS6KB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01475)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BHD-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-S6K1 (T421 + S424)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P01475 is reactive to RPS6KB1 in Human, Rat
Applications
P01475 is guaranteed for IP, WB Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
59.14kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:30
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of SK61 phosphorylation expression in HEK293 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For RPS6KB1 (Source: Uniprot.org, NCBI)
Gene Name
RPS6KB1
Full Name
Ribosomal protein S6 kinase beta-1
Weight
59.14kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.1; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 Kinase; p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase, alpha 2; p70 S6KA; p70 S6K-alpha; p70(S6K)-alpha; p70-alpha; p70-S6K; P70S6K1; PS6K; ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; RPS6KB1; S6K; S6K1; S6K1p70-S6K 1; S6K-beta-1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A; STK14A RPS6KB1 PS6K, S6K, S6K-beta-1, S6K1, STK14A, p70 S6KA, p70(S6K)-alpha, p70-S6K, p70-alpha ribosomal protein S6 kinase B1 ribosomal protein S6 kinase beta-1|ribosomal protein S6 kinase I|ribosomal protein S6 kinase, 70kDa, polypeptide 1|serine/threonine kinase 14 alpha|serine/threonine-protein kinase 14A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS6KB1, check out the RPS6KB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS6KB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-S6K1 (T421 + S424) RPS6KB1 Rabbit Monoclonal Antibody (P01475)
Hello CJ!
P01475 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu R,Zhang Y,Li A,Ma Y,Cai W,Song L,Xie Y,Zhou S,Cao W,Tang X.LY‑294002 enhances the chemosensitivity of liver cancer to oxaliplatin by blocking the PI3K/AKT/HIF‑1α pathway.Mol Med Rep.2021 Jul;24(1):508.doi:10.3892/mmr.2021.12147.Epub 2021 May 13.PMID:33982772.
Species: Human
P01475 usage in article: APP:WB, SAMPLE:HEPG-2 CELL, DILUTION:NA
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho-S6K1 (T421 + S424) RPS6KB1 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Phospho-S6K1 (T421 + S424) RPS6KB1 Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-Phospho-S6K1 (T421 + S424) RPS6KB1 Rabbit Monoclonal Antibody
Question
We are currently using anti-Phospho-S6K1 (T421 + S424) Rabbit Monoclonal antibody P01475 for rat tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on zebrafish tissues as well?
H. Krishna
Verified customer
Asked: 2014-09-26
Answer
The anti-Phospho-S6K1 (T421 + S424) Rabbit Monoclonal antibody (P01475) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-09-26
